Protein purification can be a challenging and time-consuming process. Unlike nucleic acid purification, which is often straightforward, protein purification typically involves multiple tedious steps, long incubation times, and a high risk of low yield or poor purity. Many researchers spend an entire day in the lab only to end up with disappointing results. This is why many scientists dream of a method as simple and efficient as plasmid purification.
Today, that dream has become a reality. Clontech (now part of Takara) has introduced a revolutionary new product: the His-Tagged Purification Miniprep Kit. Just like plasmid extraction, this kit allows you to load, bind, wash, elute, and centrifuge in just minutes. With this system, you can purify up to 100 μg of His-tag fusion protein in under 5 minutes—making the process fast, reliable, and highly efficient.
The kit uses a specially designed purification column equipped with a modified nylon membrane. This membrane offers a large surface area and strong binding capacity, comparable to traditional resin-based methods. However, unlike resins, the membrane allows for faster flow rates, enabling quick purification without compromising yield or purity. The column can be used at room temperature, eliminating the need for lengthy equilibration steps and making it ideal for both mammalian and bacterial cell lysates.
Even when dealing with complex lysates containing additives such as β-mercaptoethanol, EDTA, DTT, glycerin, or other common reagents, the system remains stable and performs consistently. The purification process is simple: take 2–5 ml of culture medium, prepare 200–800 μl of lysate, add it to the column, and complete the entire procedure in just 5 minutes. You'll obtain a concentrated protein solution with a typical concentration of 0.3–1 mg/ml.
This streamlined approach not only saves time but also reduces the risk of protein degradation or loss of activity, ensuring better results for downstream applications such as ELISA, Western blot, immunohistochemistry, and more.
Whether you're working with SOD kits, IgG or IgM kits, or conducting free tests, this kit provides a powerful and user-friendly solution. From Shanghai Xinfan Biological to GIBCO and AMRESCO, this technology is helping researchers across the globe achieve faster, more consistent protein purification results.
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