Protein purification has always been a challenging and time-consuming process. Unlike nucleic acid purification, which is relatively straightforward, protein isolation often involves multiple complex steps, long incubation times, and a high risk of protein degradation or loss of activity. Many researchers spend entire days in the lab only to end up with low yields and poor purity—something that can be frustrating and discouraging.
But what if you could purify proteins as easily and quickly as plasmids? That’s now a reality with the new generation His-Tagged Purification Miniprep Kit from Clontech (Takara). This innovative kit allows you to isolate up to 100 μg of His-tag fusion protein in just 5 minutes, using a simple workflow: loading, binding, washing, eluting, and centrifuging—just like plasmid purification.
The system features a specially designed purification column equipped with a modified nylon membrane. This membrane offers a large surface area and strong binding capacity, comparable to traditional resins but with faster flow rates. The column can be used at room temperature without lengthy equilibration steps, making it ideal for both bacterial and mammalian cell lysates. Even when dealing with complex samples containing additives such as β-mercaptoethanol, EDTA, DTT, glycerin, or other components, the system remains stable and efficient.
After preparing 2–5 ml of culture medium and adding it to the column, the sample is equilibrated, bound, washed, and eluted in under 5 minutes. The purified protein is concentrated, with a typical yield of 0.3–1 mg/ml. This streamlined process minimizes the risk of protein denaturation or activity loss, ensuring better results for downstream applications like Western blotting, ELISA, immunohistochemistry, and more.
Whether you're working with SOD kits, IgG or IgM detection, or looking for free testing options, this kit provides a reliable and efficient solution. From Shanghai Xinfan Biological to GIBCO and AMRESCO, this technology is supported by leading suppliers and trusted by researchers worldwide.
Try it today and experience the power of fast, efficient, and high-quality protein purification.
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